FastQCFastQC Report
Sun 14 Apr 2019
SRR6577006_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577006_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711113
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13520.1901244949818102No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11290.1587652032799288No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11240.1580620801475996No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11140.15665583388294124No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10350.1455464883921402No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10180.143155869742221No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10040.14118712497169927No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9790.1376715093100534No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9700.13640588767186088No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9430.1326090227572833No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9370.13176527499848828No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC9160.12881215784270572No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9020.12684341307218402No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8000.11249970117266875No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7990.11235907654620293No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG7750.10898408551102286No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7730.1087028362580912No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA7530.10589034372877448No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC7160.10068723254953854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14150.050.2747841
GGTATCA5200.043.4878121
CAACGCA16000.043.4681055
ATCAACG16300.042.379783
AACGCAG17650.040.2032556
TCAACGC18100.038.4248434
ACGCAGA21050.033.263137
TATCAAC22300.031.8245432
CGCAGAG21850.031.6351418
ACCTAAG4700.031.0737421
GTACATG25950.030.5001241
CCTAAGA4650.030.3220372
TACATGG26050.029.769142
ACGCCTA1751.70985E-1029.5381726
ACCGTCA804.765811E-429.388948
GCAGAGT23700.029.3641419
GGTAATC2101.8189894E-1229.1090478
ACATGGG27150.028.5590063
CTAACGC2002.7284841E-1128.1955283
CTAGTAC3350.028.0552523
Produced by FastQC (version 0.11.2)