FastQCFastQC Report
Sun 14 Apr 2019
SRR6577006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711113
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13230.18604638081430097No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12140.17071829652952486No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12000.16874955175900314No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11680.1642495637120964No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10990.15454646448595372No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10830.15229647046250033No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10620.14934335330671777No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10490.1475152331626619No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10150.14273399586282348No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9980.14034337721290427No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC9960.1400621279599726No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9130.12839028396330823No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8750.12304654815760646No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8300.11671843996664383No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8300.11671843996664383No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8220.11559344295491716No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8180.1150309444490538No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT7950.11179657804033959No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA7490.10532784522291114No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7400.10406222358471862No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7360.10349972507885527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13650.052.6923941
GGTATCA4800.051.9067461
GTACTAG1850.045.7393461
CAACGCA16300.042.9510735
ATCAACG16450.042.559423
TCAACGC17400.040.2357754
AACGCAG17650.040.198296
ACGCAGA21550.032.487367
TATCAAC21800.032.346252
CGCAGAG22000.031.8228428
GACGTTA953.757511E-529.6758387
CTAGGCA3200.029.3667184
CTAATGG650.00616109428.914923
TACATGG26600.028.8068072
GTACATG27150.028.3963951
GCAGAGT24900.028.1165689
ACATGGG28000.027.688623
GAACCGT1258.035866E-626.3125786
CATGGGG19200.025.4511534
CTAGTAC3550.025.1478373
Produced by FastQC (version 0.11.2)