FastQCFastQC Report
Sun 14 Apr 2019
SRR6577005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787486
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13440.1706697007946808No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13220.16787600033524405No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13060.16584421818292644No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12300.1561932529594177No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12150.1542884571916199No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11560.14679626050494865No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10840.13765324081951932No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10750.13651036335884068No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10600.1346055675910429No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10490.1332087173613245No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10360.13155789436256646No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10240.1300340577483282No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10150.12889118028764956No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9090.11543062352854527No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG9030.11466870522142615No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8820.11200199114650927No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT8750.11111308645487031No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8730.11085911368583061No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8310.10552568553599682No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8160.10362088976819907No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC7900.10031924377068291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15200.051.3420561
GGTATCA5650.046.5959661
ATCAACG16250.045.9848333
CAACGCA16550.044.5833325
TCAACGC17850.041.5996554
AACGCAG18150.041.429936
ACGCAGA21100.034.969397
CGCAGAG22450.032.8665548
TATCAAC23800.032.7898862
GTACATG29450.031.4477581
GTACTAG1950.031.3413351
CCGTATA600.004161458531.33138894
TACATGG29400.031.181432
GCAGAGT24400.030.6251459
ACATGGG30550.030.1519723
CTAACGC1704.0745363E-927.645343
ACCTAAG5700.027.2174781
ACGCCTA1406.3675E-726.8554746
CATGGGG22250.026.8252984
GTATAAG2156.548362E-1126.239261
Produced by FastQC (version 0.11.2)