FastQCFastQC Report
Sun 14 Apr 2019
SRR6577004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431104
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19820.459749851543943No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15890.3685885540380047No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12610.29250482482185275No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8280.1920650237529691No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7760.18000296912114014No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7200.16701306413301661No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6500.15077568289786222No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA5530.1282753117577197No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT5220.12108447149643704No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5110.11853288301662708No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA4940.1145895190023753No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC4700.10902241686460808No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA4570.10600690320665083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.10484709026128267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2800.067.14891
GTATCAA11350.050.9385071
ATCAACG11600.048.6081543
TCAACGC11600.048.6081544
CAACGCA12250.046.412525
TATCAAC12550.044.9442862
AACGCAG12800.044.418236
CGCATTA250.001703948837.590370-71
GTCTTAG4650.037.401221
ACGCAGA16000.035.5345847
TTAGGTA4100.035.527424
CTTAGGT4250.035.379113
TAGGTAT4150.035.099385
CGCAGAG16650.034.4295588
AGGTATA4500.032.369436
TACATGG19150.031.908882
ATATGAG1351.3264071E-831.3252543
AACCGTC1052.0655625E-631.3252537
ACCGTCA600.004163375631.3252538
GCAGAGT18800.030.9920049
Produced by FastQC (version 0.11.2)