FastQCFastQC Report
Sun 14 Apr 2019
SRR6577003_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577003_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457050
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21330.4666885461109288No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14660.3207526528826168No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11360.24855048681763484No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8150.1783174707362433No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7540.1649710097363527No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG6640.14527950990044855No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6030.1319330489005579No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT5540.12121212121212122No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5200.11377311016300186No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA5130.11224154906465376No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT4860.10633409911388252No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC4650.10173941581883822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3450.062.7884141
GTATCAA10450.050.471071
ATCAACG11800.044.2105263
TCAACGC12100.043.5028154
CAACGCA12250.042.970135
AACGCAG12450.041.9023486
TATCAAC13750.037.940672
CCATGCG651.4124095E-436.1527529
GTCTTAG5000.035.7893941
CTTAAGT1451.8189894E-1135.654093
TTAGGTA4300.034.9756854
CTTAGGT4300.034.9756853
CGCAGAG15800.032.7205288
TAGGACA1457.421477E-1032.412814
TCTTAGG5950.032.3855742
GGTATAG4500.032.3767977
ACGCAGA16200.031.9126157
TCTAGAT1807.2759576E-1231.3323862
TTAAGTA2100.031.3323864
TACTACG600.004158999331.3323862
Produced by FastQC (version 0.11.2)