FastQCFastQC Report
Sun 14 Apr 2019
SRR6577003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457050
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20420.4467782518324035No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15080.3299420194727054No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13200.2888086642599278No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8700.19035116508040695No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8060.1763483207526529No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7440.16278306531014114No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6480.14177879881851002No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA6140.13433978776939065No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT5990.13105787113007328No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5640.12340006563833279No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT5140.11246034350727492No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA4960.10852204354009408No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA4760.10414615468767094No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG4710.10305218247456516No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC4670.10217700470408053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3200.061.697771
GTATCAA12200.050.860921
TCAACGC12950.046.0902524
CAACGCA13250.045.4013945
ATCAACG13300.044.8773463
AACGCAG13650.043.7266466
TATCAAC14700.040.931982
ACGCAGA16100.036.780687
CGCAGAG16400.036.3944328
TACATGG20250.033.195652
GTACATG20700.032.701091
ACATGGG20950.032.30383
GCAGAGT18800.031.7483399
TAGGTAT5150.031.0275025
CTTAGGT5150.031.0275023
GGTAATC1859.094947E-1230.4848888
GTCTTAG5750.030.2485121
TTAGGTA5550.029.6380884
ACCAGAT8100.029.59104394
AGGTATA5600.028.5342226
Produced by FastQC (version 0.11.2)