FastQCFastQC Report
Sun 14 Apr 2019
SRR6576997_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576997_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833939
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26890.3224456465041208No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18590.2229179832098031No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15880.19042160157997168No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11430.1370603845125363No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11110.13322317339757464No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8790.10540339281410271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15600.055.8348241
GGTATCA3250.052.1527441
ATCAACG16900.049.216473
TCAACGC16900.049.216474
CAACGCA17900.046.729475
AACGCAG18850.044.3770646
TATCAAC20500.041.261262
CTTAGGT4700.036.99373
TTAGGTA5150.035.586174
GTCTTAG6000.035.3117561
TACATGG33300.034.997042
GTACATG33850.034.6336821
ACGCAGA24250.034.4951637
TAGGTAT5250.034.0132525
CGCAGAG24800.033.9399958
GGTATAG5150.033.7632647
AGGTATA5300.032.80776
ACATGGG35350.032.169913
GTATAAC600.00412484331.3882261
ATTATCC10800.031.3279973
Produced by FastQC (version 0.11.2)