FastQCFastQC Report
Sun 14 Apr 2019
SRR6576994_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576994_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966472
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43040.4453310597720368No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28330.293127995430804No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25460.2634323601718415No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16070.16627486362770985No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15090.15613489061245436No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT12460.12892251405110547No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10660.11029807381900356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17650.054.2156521
GGTATCA4600.047.1382371
TCAACGC19700.046.5220764
ATCAACG20150.045.2498743
CAACGCA20800.044.0617685
AACGCAG22400.041.334146
TATCAAC23200.039.3010752
GTACATG37350.034.706871
TACATGG37300.034.65092
ACATGGG38800.033.190173
ACGCAGA27800.032.9653747
CGCAGAG28950.031.6558698
TTAGGTA9600.031.3328174
ACCTAAG14650.030.8892231
GGTAATC2900.030.7918
GTCTTAG11150.030.4390431
TAGGTAT9600.030.3536665
CTTAGGT9700.030.040743
ACCAGAT17150.029.60177294
CTAAGAC16400.029.2312243
Produced by FastQC (version 0.11.2)