FastQCFastQC Report
Sun 14 Apr 2019
SRR6576991_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576991_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43134
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.3964390040339407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.23183567487364956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.22256224787870357No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA650.1506931886678722No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC560.12982797792924375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTG201.818813E-593.989565
TAGTACT156.859869E-493.9895554
GCCTAGT200.002130039770.6561051
TAATGCT200.002149788470.492174
CCTAGTA250.005204296756.3937382
GGGAGAT250.005204296756.3937387
CTAGTAC250.005204296756.3937383
ACCGTGG250.005204296756.3937386
GTACTGT352.907428E-453.708326
GTATCAA1800.047.104071
ATGGGAG708.360075E-846.994785
GTGCGCC205.650471E-446.9947810-11
CATGGGA1350.041.7731364
ACATGGG2900.040.512743
TACATGG2950.039.8260842
AACGCAG2200.038.4502756
GTACATG3100.037.9871521
CACGGGA250.001694232837.59582586-87
CATTGCG250.001694232837.59582528-29
CAACGCA2350.035.9960025
Produced by FastQC (version 0.11.2)