FastQCFastQC Report
Sun 14 Apr 2019
SRR6576982_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576982_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533028
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA38810.728104339734498No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20600.3864712547933692No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19310.36226989951747374No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13000.24388962681134949No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11130.20880704203156308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.1907967311285711No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10040.1883578348604576No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8390.1574026129959402No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA8340.1564645759697427No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG7770.1457709538710912No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA7460.13995512430866672No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7350.13789144285103222No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT7190.13488972436720023No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT7120.1335764725305237No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT7030.13188800588336821No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG6500.12194481340567474No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC6420.12044395416375875No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA6200.11631659124848977No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC6160.11556616162753175No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA6020.11293965795417876No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT5990.11237683573846026No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC5700.10693622098651477No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5650.10599818396031728No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5430.10187082104504828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4000.062.328941
GTATCAA18100.053.5380441
TCAACGC19100.049.9445574
ATCAACG19350.049.2992743
CAACGCA19600.048.6704565
AACGCAG20600.046.535936
TATCAAC20600.046.3078162
ACGCAGA24700.038.8113447
CGCAGAG25050.038.2978138
GTATAGA500.001693988537.632571
GCAGAGT27050.035.2923249
GTACATG29300.035.160121
GGTAATC2950.033.4771778
CTAGACC851.755025E-533.170944
TACATGG31550.032.4700242
ACATGGG31100.032.3354453
CATGGGG14250.032.317424
GTGCAAG3700.030.5128921
AGGTATA6000.029.7617076
CTTAGGT6050.029.5157433
Produced by FastQC (version 0.11.2)