FastQCFastQC Report
Sun 14 Apr 2019
SRR6576982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533028
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA35060.6577515627696856No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21310.39979138056537367No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18850.3536399588764568No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16590.31124068529232984No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13020.24426484162182852No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA11130.20880704203156308No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10950.2054301087372521No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10480.19661256069099559No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9710.1821667904875541No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT8140.15271242786495268No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT7940.14896027976016268No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC7500.14070555392962472No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG7250.1360153687986372No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7080.1328260429095657No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA6990.13113757626241024No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT6800.12757303556285973No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT6400.12006873935327975No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT6310.11838027270612426No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC6140.11519094681705275No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT6070.11387769498037628No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA6020.11293965795417876No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA5850.10975033206510726No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA5700.10693622098651477No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG5660.1061857913655568No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA5590.10487253952888029No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA5510.10337168028696428No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG5500.10318407288172479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3900.061.454361
GTATCAA18800.053.493751
TCAACGC20150.049.900444
ATCAACG20300.049.531723
CAACGCA20450.049.1684075
TATCAAC21050.047.3293882
AACGCAG21700.046.336126
ACGCAGA25650.039.2005427
CGCAGAG26050.038.4182478
GCAGAGT28050.035.6789749
GTACATG30200.034.3900221
ACATGGG30900.033.6046563
TACATGG31400.032.926092
TACGTGT1052.0675234E-631.3237974
CTAAGAC12650.031.1999863
TAGGTAT6050.031.0649225
CTTAGGT6150.030.5598013
ACCTAAG12450.030.1972731
CCTAAGA12950.029.394252
CATGGGA17500.029.2653734
Produced by FastQC (version 0.11.2)