FastQCFastQC Report
Sun 14 Apr 2019
SRR6576981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584653
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA37200.6362748502102957No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22310.3815938684997768No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21010.3593584570676966No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16960.29008659837544665No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13940.23843202720246026No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12440.2127757832423677No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA11790.20165807752632758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11600.19840828662471585No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10800.1847249565126665No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9440.16146329532218257No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG8700.1488062149685369No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT8510.14555642406692515No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8150.13939892551650296No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT8070.138030592505298No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC7890.1349518432300869No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA7850.13426767672448445No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC6740.11528205619401595No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT6520.11151914041320236No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT6500.11117705716040113No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT6460.11049289065479867No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA6380.10912455764359372No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG6190.105874766741982No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA6150.10519060023637954No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG6040.10330914234597274No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA5890.10074351794996349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3550.059.5850561
GTATCAA19700.053.9256551
TCAACGC21250.048.4355284
ATCAACG21300.048.3218353
CAACGCA21800.047.2135355
AACGCAG22350.046.0516786
TATCAAC22900.045.5691222
TCTAGAC704.632724E-640.2839553
ACGCAGA26750.038.476827
CGCAGAG26950.038.191288
GGTAATC4350.036.7340288
GCAGAGT29700.034.8132939
GTGCAAG5200.034.350531
CTTAGGT6450.034.2465673
AGGTATA6550.033.723726
TAGGTAT6750.033.420765
AAGGGTA4950.033.2308735
TTAGGTA6950.033.1352424
GTCTTAG7250.033.066281
GGTATAG6700.032.9687087
Produced by FastQC (version 0.11.2)