FastQCFastQC Report
Sun 14 Apr 2019
SRR6576979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660126
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14250.21586787976840782No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12410.18799441318778537No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10330.15648527705316864No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7830.118613719199062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4000.065.853651
GTATCAA13450.059.1039241
ATCAACG17300.045.3622253
TCAACGC17300.045.3622254
CAACGCA18300.042.8834155
AACGCAG19000.041.550836
TATCAAC19850.039.5378342
CGCAGAG24450.032.1186878
ACGCAGA24650.031.8363727
GTCTTAG4350.031.3588851
CTAACGT300.004164668331.32800552-53
GTACATG29450.031.3056451
TACATGG29950.031.225772
ACATGGG30950.029.4554123
GCAGAGT26850.029.422889
ACCTAAG6250.027.8466911
TAGGTAT3750.027.5686455
TTAAGTA2253.6379788E-1227.150944
CTTAGGT3850.026.8525773
TCGCCCG700.00884067426.8525755
Produced by FastQC (version 0.11.2)