FastQCFastQC Report
Sun 14 Apr 2019
SRR6576979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660126
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13530.20496087110642514No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12930.19587169722143954No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10940.16572593716957068No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8250.12497614091855191No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7140.10816116923132855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3400.064.972631
GTATCAA12400.060.6470641
TAGGCCG451.6157921E-552.2080425
TCAACGC15900.046.1006854
ATCAACG16150.045.3870543
CAACGCA17150.042.7405785
AACGCAG17300.042.369996
TATCAAC18750.039.8572542
TACATGG24850.035.180182
GTACATG25000.034.96911
CGCAGAG21600.034.3702938
GTCTTAG4850.033.918591
ACGCAGA21650.033.8568537
GGTAATC1703.6379788E-1233.167468
ACATGGG26600.032.8557363
TAGGTAT4000.031.7163835
CTTAGGT4050.031.3248253
GGTATAG4250.030.9562977
TTAAGAC3500.030.8773273
AGGTATA4150.030.570016
Produced by FastQC (version 0.11.2)