FastQCFastQC Report
Sun 14 Apr 2019
SRR6576978_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576978_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752680
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15120.20088218100653665No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13810.183477706329383No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10630.14122867619705584No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8680.11532125205930809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4850.058.3052441
GTATCAA14800.057.63881
TCAACGC18500.045.2202454
ATCAACG19150.043.930783
CAACGCA19300.043.3458335
AACGCAG19750.042.3582086
TATCAAC22750.037.1856842
ACGCAGA26100.032.0526667
CGCAGAG26600.031.626868
GTACATG31950.031.5675581
TACATGG31950.030.8910792
GGTAATC1401.8897481E-830.2133678
ACATGGG32650.029.9408973
CTTAGGT3500.029.5419583
ACCTAAG6450.029.2279531
GCAGAGT29150.028.8601869
GTCTTAG4100.028.7378481
TAGGTAT3450.027.2455485
CTAAGAC7350.026.8563273
GTGTAAT2007.858034E-1025.921541
Produced by FastQC (version 0.11.2)