FastQCFastQC Report
Sun 14 Apr 2019
SRR6576958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48915
Sequences flagged as poor quality0
Sequence length100
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.33527547786977413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.15741592558519882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.12266176019625882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG255.4852146E-575.258131
CAAACGC200.00214398670.5545042
GTATCAA2500.056.4436041
TGGGAAC250.00521135556.3858876
CATGGGG1350.048.7285464
ATCAACG2900.048.6085243
GGTATCA902.382876E-1047.036341
ATGGGGT601.5763089E-646.988245
CAACGCA3050.046.217945
AACGCAG3050.046.217946
TCAACGC3050.046.217944
TATCAAC3350.042.122092
CGCAGAG3350.042.079028
GTACATG4250.040.949281
ACATGGG4300.040.4317443
TACATGG4200.040.316862
GCAGAGT3500.040.2756359
ACGCAGA3550.039.708377
TTGGATG500.001688216237.590594
ATGGGGG550.002695069634.1732645
Produced by FastQC (version 0.11.2)