FastQCFastQC Report
Sun 14 Apr 2019
SRR6576957_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576957_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704074
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44600.6334561423941234No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC25230.3583430150808012No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24730.3512414888207774No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18060.2565071285120598No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG13500.19174120902064273No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12660.17981064490380272No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11850.16830617236256418No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT11450.1626249513545451No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10780.15310890616611322No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC10610.15069438723770512No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC10160.1443030136036837No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10080.1431667694020799No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9410.133650724213648No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9280.1318043273860418No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC9180.13038402213403705No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT8470.12029985484480325No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8190.1163230001391899No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8140.11561284751318754No Hit
GTATAAATTCTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAAT8110.11518675593758612No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT7650.10865335177836422No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA7620.10822726020276278No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA7580.10765913810196087No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA7510.10666492442555754No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG7200.10226197814434278No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACA200.002162980470.497122
GGTATCA4150.069.2093351
GTATCAA19300.051.7203521
ATCAACG21400.045.4543043
TATCAAC21400.045.2411462
TCAACGC21350.045.1205564
CAACGCA21500.044.805765
AACGCAG22100.043.5893146
GTGCAAG3750.037.6680341
ACGCAGA26200.036.7706957
ATAGTAC651.4144536E-436.1472363
CGCAGAG27250.035.5464978
AAGGGTA3600.035.2435535
GGTAATC3500.033.5867588
TACATGG33000.033.3259122
GTACATG32650.033.3128471
ACATGGG33500.032.823853
GCAGAGT29650.032.6692129
ATGGGGG6100.030.8140375
CTGGTCG1701.2187229E-1030.4256529
Produced by FastQC (version 0.11.2)