FastQCFastQC Report
Sun 14 Apr 2019
SRR6576955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007941
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA38530.38226443809707117No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20800.20636128503553286No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17210.17074412093565No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12290.12193174005224514No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12090.11994749692690347No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT11940.11845931458289723No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10410.1032798546740335No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10390.10308143036149933No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.058.4917071
GTATCAA23750.055.0328641
ATCAACG28000.045.8105353
TCAACGC28250.045.4051324
GGTAATC2750.044.422348
CAACGCA29050.044.154735
AACGCAG29750.043.273736
TATCAAC34450.037.5305062
ACGCAGA36750.034.9032677
CGCAGAG37850.033.8889058
AGGGTAA3800.033.3841976
GTACATG45850.031.8905491
GCAGAGT40400.031.7498789
TACATGG45400.031.6888542
ACCTAAG13450.031.4601761
CTAAGAC14100.030.6569863
AAGGGTA4150.030.5686635
ACATGGG47350.030.2649963
TAAGACA16050.030.1524754
CATGGGG26750.027.9276314
Produced by FastQC (version 0.11.2)