FastQCFastQC Report
Sun 14 Apr 2019
SRR6576954_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576954_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005232
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA35830.35643513139255417No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19730.1962730991452719No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16160.16075890938609197No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12130.12066866156270394No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11030.10972591401785857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5600.063.9642031
GTATCAA20300.055.489811
TCAACGC27150.040.691714
ATCAACG27900.039.5978553
CAACGCA28400.039.0642975
AACGCAG29000.038.256076
TATCAAC31750.034.944292
ACGCAGA35200.031.2429127
GTACATG44800.030.4040371
TACATGG44950.030.2256832
AGGTATA7000.030.2204446
TAGGTAT7050.030.0061115
GTCTTAG7550.029.9644071
TTAGGTA7250.029.8282494
CGCAGAG36950.029.6360158
CTTAGGT7300.029.623953
ACCTAAG11200.029.45721
GCGCGAC804.7817206E-429.373689
ACATGGG46000.029.2291493
CTAAGAC13250.028.0297473
Produced by FastQC (version 0.11.2)