FastQCFastQC Report
Sun 14 Apr 2019
SRR6576952_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576952_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918448
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA49290.536666202114872No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21330.23223960420187098No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16360.17812657875023952No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16000.17420692298311935No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15220.16571433548769227No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG14360.15635071337734963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14010.1525399369370939No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC11050.1203116561852168No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10990.11965838022403011No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA10870.11835182830165672No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10160.11062139609428079No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10070.10964148215250075No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10050.10942372349877184No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC9690.10550406773165166No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9650.10506855042419386No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9410.10245544657944707No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9410.10245544657944707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6600.065.726061
GTATCAA28450.057.17821
ATCAACG35800.045.4293673
TCAACGC36300.044.8036234
CAACGCA36800.044.4503365
AACGCAG38350.042.6537786
TATCAAC39850.041.4021032
ACGCAGA44350.036.7712677
AAGGGTA3850.036.627245
TAGGTAT6700.036.4814575
CGCAGAG45850.035.7752278
TTAGGTA7100.035.0882034
CTTAGGT7250.034.3622443
GCAGAGT47900.034.2441379
GGTATAG7150.034.179847
GGTAATC3950.033.3163388
GTCTTAG8050.032.8009071
GTATAGT7900.032.7214058
AGGTATA7600.032.1612856
GTGCAAG5400.030.561031
Produced by FastQC (version 0.11.2)