FastQCFastQC Report
Sun 14 Apr 2019
SRR6576952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918448
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA46220.5032402487674861No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20190.21982736093932373No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16670.18150183788303748No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16290.17736442346218836No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15980.17398916432939043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.1343570893507308No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA11850.12902200233437278No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11410.12423131195233698No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10620.11562984513004547No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10000.1088793268644496No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9930.10811717157639844No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9900.1077905335958051No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC9870.10746389561521175No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG9800.1067017403271606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8300.066.834411
GTATCAA30400.056.7529831
TCAACGC36300.046.2210244
ATCAACG36450.046.1597563
CAACGCA37700.045.0032465
AACGCAG38250.044.6018756
TATCAAC40400.042.1233982
AAGGGTA4100.037.8275385
ACGCAGA46000.037.0874337
CGCAGAG47250.036.205758
GCAGAGT49250.034.5446139
CTTAGGT6850.033.61893
GTCTTAG8600.033.344761
TAGGTAT6750.032.724435
GTGCAAG5900.032.6684151
TTAGGTA7350.031.9713274
GTACATG55000.031.5398711
GGTATAG7300.030.9026977
AATAGTA3650.030.9026975
AGGTATA7350.030.6924746
Produced by FastQC (version 0.11.2)