FastQCFastQC Report
Sun 14 Apr 2019
SRR6576947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202552
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5060.24981239385441764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.2350013823610727No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3430.16933923140724358No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3040.15008491646589517No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2610.1288557999921008No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2570.1268809984596548No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2430.11996919309609384No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2410.11898179232987084No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2410.11898179232987084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.11552588964809038No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2210.10910778466764091No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT2190.10812038390141791No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2150.10614558236897192No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2150.10614558236897192No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2120.10466448121963742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6900.053.876681
GCTACCG352.9321053E-453.712879
ATCAACG8750.041.8960463
TCAACGC9250.039.6313934
CAACGCA9300.039.4183245
AACGCAG9550.038.878566
CATATAG757.414652E-637.599013
GGTACCT1850.035.5666358
GACATAT801.1444252E-535.2926371
TATCAAC10650.034.464172
ATGGGAT3100.033.3539625
ACCTAAG851.7332815E-533.21661
ACGCAGA11050.033.17567
GTACATG14000.032.6036721
GGTATCA2600.032.5778161
CGCAGAG11600.031.602628
GCAAACG600.00412846131.371231
GTACTTG1201.6235936E-731.371231
ATAGGCG300.004158365531.3325148-49
CGACGGT600.00415350231.332517
Produced by FastQC (version 0.11.2)