FastQCFastQC Report
Sun 14 Apr 2019
SRR6576945_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576945_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782661
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28220.36056479114201423No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18370.23471209118635017No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17970.2296013216450034No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11390.1455291626898491No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8890.11358685305643183No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT8210.10489854483614235No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8160.104259698643474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.1001710830103966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3500.061.9330751
GTATCAA15800.056.666891
TCAACGC18850.046.382144
ATCAACG18900.046.010733
CAACGCA19950.044.0575335
AACGCAG20200.043.744956
GTATAGG2050.039.0776021
TATCAAC22250.038.8720132
ACGCAGA24200.036.3178377
CGCAGAG25200.034.6901448
GTACATG30500.033.2178461
TACATGG31350.032.2366682
GCAGAGT27300.032.1917769
ACATGGG31800.031.7804893
GTCTTAG7600.031.0019681
TAGGTAT6700.030.165825
GGGTACC4450.028.5165827
ATTATCC12250.028.3951953
AGGTATA7100.027.8043276
TATTATC13050.027.735082
Produced by FastQC (version 0.11.2)