FastQCFastQC Report
Sun 14 Apr 2019
SRR6576935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267780
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.2098737769811039No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT200.00215350870.563091
GGACCGT250.00523248956.397766
GTATCAA8700.044.8792041
GACCGTG608.808015E-539.165117
TACATGG17600.032.317252
GTACATG18100.031.9678061
ATGGGCG600.00415614431.332095
ATCAACG12300.031.3320883
CAACGCA12500.030.8307745
ACATGGG17700.030.8010373
TCAACGC12550.030.7079434
GTACTTG1701.200533E-1030.4389781
AACGCAG12850.029.9910286
CATGGGT2550.029.4890234
TATCAAC13150.029.3122942
GATATGC650.006147048428.9219263
GTAAGGC650.006147048428.9219263
GGTATCA4500.028.2252331
GTATAGG700.00878572426.8811741
TGGGACG700.00882585126.8560776
Produced by FastQC (version 0.11.2)