FastQCFastQC Report
Sun 14 Apr 2019
SRR6576932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097329
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA173671.5826611708976979No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC117471.0705084801367684No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC114831.0464500619230879No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG61180.5575356160276452No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC56890.5184406864304142No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT51610.4703238500030528No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC50250.4579301194081265No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG47800.4356031782628546No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT46510.42384736027207887No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA44200.4027962443351083No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT43670.3979663346179678No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC39730.36206096804148985No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA39420.35923592650882274No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA38240.3484825426102837No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT38240.3484825426102837No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT37380.34064533061643315No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA36620.33371942234279783No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT36050.32852499113757133No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA36030.3283427303935283No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA35940.3275225570453346No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG35830.32652012295309796No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT34900.31804499835509675No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT33470.3050133551560198No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT29280.26682972927900384No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG27090.24687217780629145No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA26170.23848818358031182No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC25320.23074210195848283No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA25240.23001305898231067No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG23590.21497654759876028No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG22100.20139812216755415No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC22060.20103360067946804No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA21740.19811742877477947No Hit
CTCTTACAGTATGGAATTTAAGAACTAAGGAATTCTCCAAGACATGGATT21590.1967504731944567No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA21500.19593029984626306No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA21440.19538351761413394No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA21370.19474560500998334No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG21310.19419882277785422No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG21080.19210282422135933No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT20990.19128265087316568No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT20830.18982456492082136No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG20660.18827534859645556No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA20260.18463013371559486No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC20250.18453900334357334No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG20020.18244300478707845No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG19930.1816228314388848No Hit
GTATAAATTCTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAAT19740.17989135437047596No Hit
GTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT19660.1791623113943038No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA19420.1769751824657874No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC19020.17332996758492666No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA19000.17314770684088365No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT18980.17296544609684059No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT18970.1728743157248191No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCACCTCATTAACGAAA18740.17077831716832417No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC18450.16813553637970016No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA18360.16731536303150651No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC18170.16558388596309767No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA18050.16449032149883946No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC17790.16212093182628No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA17350.1581111954573332No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC17200.15674423987701044No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC16720.1523699820199776No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT16480.15018285309146118No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG16250.14808685453496628No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA16180.14744894193081565No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG16150.1471755508147511No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC16110.146811029326665No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA16090.14662876858262197No Hit
CTCCAAGACATGGATTTGCTCCAGAAAAAGTCTAAAGTTGCCATTATCTT16060.14635537746655744No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC15770.1437125966779334No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA15650.14261903221367522No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT15480.14106981588930942No Hit
GGGTATATCAAAGAAAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT15160.13815364398462082No Hit
CCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCAGAC14960.1363310365441905No Hit
ACCATAGATAATTCGGAGCACTCTTCATCTCTTACAGTATGGAATTTAAG14870.13551086319599684No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGAACATGGCAAC14620.13323260389545888No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT14490.13204790905917915No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC14070.12822043343427542No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC14070.12822043343427542No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT14050.12803817269023238No Hit
GTCTTGGAGAATTCCTTAGTTCTTAAATTCCATACTGTAAGAGATGAAGA14000.12758252083012478No Hit
CTCTTCATCTCTTACAGTATGGAATTTAAGAACTAAGGAATTCTCCAAGA13770.12548652227362989No Hit
GAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAG13720.1250308704135223No Hit
ATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTG13650.12439295780937168No Hit
GGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGC13620.1241195666933071No Hit
CCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAAC13600.12393730594926408No Hit
CAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTG13580.12375504520522104No Hit
GGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGA13500.1230260022290489No Hit
ATTCCATACTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTG13380.12193243776479068No Hit
GTATGATGGATTCAATACATTTACTATACCTAAGACAGACTATGATAACT13250.12074774292851097No Hit
TAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTT13210.12038322144042489No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.11928965697616668No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC12940.11792270139584392No Hit
GGCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAAT12890.11746704953573632No Hit
TCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATG12600.11482426874711231No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA12320.11227261833050982No Hit
GTATCAACGCAGAGTACATGGGGGAGTGTAGCCACGATCACAAGAAAGAC12190.11108792349423008No Hit
AGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGG12180.11099679312220857No Hit
GGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACA12090.1101766197740149No Hit
ATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGT11760.10716931749730482No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT11680.10644027452113268No Hit
GTATGGAATTTAAGAACTAAGGAATTCTCCAAGACATGGATTTGCTCCAG11660.10625801377708965No Hit
CTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACCTTCCAGC11520.1049821885687884No Hit
AATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCC11460.10443540633665929No Hit
CTGTAAGAGATGAAGAGTGCTCGGAATTATCTATGGTTGCTGACAAAACA11450.10434427596463776No Hit
TCCCCATCCTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTC11380.10370636336048715No Hit
TCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAGAGCCCCATCAG11280.10279505964027197No Hit
TCACAGAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTG11270.10270392926825045No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATTGGC11230.10233940778016437No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT11080.1009724521998416No Hit
CATTAACGAAAAGGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCC11040.10060793071175553No Hit
GTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAG11010.100334539595691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5650.074.8700561
ATCAACG33000.045.847693
GTGCAAG10100.045.14031
TCAACGC33700.045.034794
CAACGCA34150.044.441365
AACGCAG35050.043.3002136
TATCAAC35000.043.241612
GTATCAA37250.043.1532861
AAGGGTA11600.040.505835
TGCAAGG11150.040.046342
GGTAATC12750.037.2208868
GTAATCA12800.037.0754939
AGGGTAA12700.036.997456
ACGCAGA41550.036.526417
CGCAGAG42900.035.376988
TAGGTAT32800.034.667065
TCTTAGG34150.034.270682
TTAGGTA33200.034.249394
GCAGAGT44450.034.249079
ACCGTCC550.0027207134.172198
Produced by FastQC (version 0.11.2)