FastQCFastQC Report
Sun 14 Apr 2019
SRR6576931_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576931_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105473
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA168271.522153865358991No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC114321.0341274730364287No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC110260.9974011124649811No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG55230.49960514639434883No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC52500.4749098349756168No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT48990.44315872029438985No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG48630.43990219571169986No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC47110.42615242525145347No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG44380.4014571138327214No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT42850.3876168843562891No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA37910.3429301303604882No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA37840.3422969172471874No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT37430.3385880975835683No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT36530.3304467861268434No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC36230.3277330156412685No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA35860.32438603204239275No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT35670.3226673107348619No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA35230.3186871140226853No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA34100.30846524519368634No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA33440.30249495012542144No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT33170.30005255668840397No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC31130.2815989173864943No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT29980.27119613052512365No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT28980.26215022890654044No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT28050.25373754040125807No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG27700.250571474834754No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA24820.22451927817323442No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG24290.21972495031538536No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA23750.21484016344135043No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA22590.20434691756379395No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA21670.19602468807469742No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG21500.1944868847995383No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT21300.19267770447582167No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC21090.19077806513591922No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG20970.1896925569416892No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA20800.18815475366653006No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT20400.1845363930190968No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA20150.182274917614451No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA20110.18191308154970767No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC19570.17702829467567277No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA19150.17322901599586782No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG19110.1728671799311245No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA18830.1703343274779212No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA18820.1702438684617354No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG18710.16924881928369123No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG18690.16906790125131957No Hit
CTCTTACAGTATGGAATTTAAGAACTAAGGAATTCTCCAAGACATGGATT18610.16834422912183292No Hit
GTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT18590.16816331108946125No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT18500.16734917994378876No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA18260.1651781635553288No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC17770.16074567176222304No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC17620.15938878651943558No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT17380.1572177701309756No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCACCTCATTAACGAAA17350.15694639308241812No Hit
GTATAAATTCTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAAT17220.15577042587200232No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG16550.14970967178755157No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC16490.14916691769043658No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA16420.14853370457713577No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA16320.14762911441527743No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT16150.1460913111401183No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC15970.1444630488487733No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC15820.14310616360598585No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC15640.14147790131464089No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT15140.1369549505053493No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG14950.1352362291978185No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGAACATGGCAAC14910.13487439313307514No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC14650.13252245871224352No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT14630.13234154067987186No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC14570.13179878658275687No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC14570.13179878658275687No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG14370.12998960625904024No Hit
CCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCAGAC13940.12609986856304947No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC13860.12537619643356282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13840.12519527840119116No Hit
ACCATAGATAATTCGGAGCACTCTTCATCTCTTACAGTATGGAATTTAAG13770.12456206528789035No Hit
GAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAG13600.1230242620127312No Hit
GGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGC13530.12239104889943038No Hit
GTATGATGGATTCAATACATTTACTATACCTAAGACAGACTATGATAACT13520.12230058988324455No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA13480.12193875381850121No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13460.12175783578612955No Hit
CTCCAAGACATGGATTTGCTCCAGAAAAAGTCTAAAGTTGCCATTATCTT13400.12121508168901457No Hit
GTATCAACGCAGAGTACATGGGGGAGTGTAGCCACGATCACAAGAAAGAC13270.12003911447859876No Hit
GGGTATATCAAAGAAAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT13210.11949636038148376No Hit
CTCTTCATCTCTTACAGTATGGAATTTAAGAACTAAGGAATTCTCCAAGA13120.11868222923581127No Hit
TAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTT12990.11750626202539546No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA12850.11623983579879382No Hit
CCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAAC12750.1153352456369355No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC12690.11479249153982052No Hit
GTCTTGGAGAATTCCTTAGTTCTTAAATTCCATACTGTAAGAGATGAAGA12670.11461157350744886No Hit
GGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGA12300.11126458990857307No Hit
ATTCCATACTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTG12270.11099321286001558No Hit
GGCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAAT12190.11026954073052891No Hit
TCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATG11750.10628934401835231No Hit
TTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATT11730.10610842598598065No Hit
CAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTG11690.10574658992123734No Hit
ATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTG11280.10203777025761823No Hit
TCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACCTTCCAGCTGA11250.10176639320906074No Hit
GTATGGAATTTAAGAACTAAGGAATTCTCCAAGACATGGATTTGCTCCAG11230.10158547517668907No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA11200.10131409812813158No Hit
CTGTAAGAGATGAAGAGTGCTCGGAATTATCTATGGTTGCTGACAAAACA11080.10022858993390159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7700.068.580531
GTGCAAG7850.053.455681
TGCAAGG8600.048.641552
AAGGGTA9150.047.2587855
ATCAACG35100.044.8594063
TCAACGC35450.044.549094
CAACGCA36200.043.4962775
GTATCAA37800.043.0329251
GGTAATC10000.042.7659658
TATCAAC37050.042.4983862
AACGCAG37150.042.383996
GTAATCA10650.040.155849
AGGGTAA11450.037.355266
GCAAGGG11350.036.4420363
TAGGTAT31350.036.432135
TTAGGTA31900.035.951334
CTTAGGT31950.035.453743
ACGCAGA44550.035.338987
AGGTATA32250.035.123936
GTCTTAG32700.035.0374151
Produced by FastQC (version 0.11.2)