FastQCFastQC Report
Sun 14 Apr 2019
SRR6576921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038865
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12690.12215254147555264No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12310.11849470335414129No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10820.10415212756229154No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10720.10318953858297276No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10580.10184191401192647No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10550.10155313731813084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25500.056.961611
GGTATCA9750.050.140961
ATCAACG32650.044.0366253
CAACGCA34100.042.1640975
TCAACGC34450.041.8721164
AACGCAG35600.040.7834746
TATCAAC37550.038.8056342
ACGCAGA42350.033.950317
CGCAGAG43000.033.327848
GCAGAGT48450.029.6758639
TACATGG53100.029.0350322
GTACATG54950.028.4865911
ACATGGG53500.028.3677253
GTACTAG3450.027.2505231
AGAGTAC44700.025.01757410-11
GGGTACC5950.024.4805137
CATGGGG28250.024.1171114
CATGGGA27750.022.5197934
ATGGGAG10250.022.0035675
CATGGGT11250.021.7183384
Produced by FastQC (version 0.11.2)