FastQCFastQC Report
Sun 14 Apr 2019
SRR6576913_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576913_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54120
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.4490022172949002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.20325203252032523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.1385809312638581No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTGT200.002153418770.486838
TATAATG200.002153418770.486832
GTATCAA1850.061.2446021
GTTCAGG250.00511822156.651251
GATAACA250.00521304256.3894694
AATAGTG250.00521304256.389467
GGTATCA601.5290516E-647.2093771
ATGGGAT902.419256E-1046.991225
TACATGG5350.037.7686422
GTACATG5350.037.0615651
ACATGGG5650.036.5949333
ATGGGGT651.3973046E-436.1470955
AACGCAG3250.036.147095
CATGGGA3000.036.0266044
CAACGCA3400.034.552374
TATCAAC3350.033.665352
ATCAACG3350.033.665353
TCAACGC3600.032.632794
CATGGGG2350.031.9940244
TGGGATT902.5521409E-531.3274786
Produced by FastQC (version 0.11.2)