FastQCFastQC Report
Sun 14 Apr 2019
SRR6576911_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576911_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931119
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA31890.3424911316383835No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17010.18268341640542185No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14360.1542230370124549No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9640.10353134239554772No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9520.1022425704985077No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG9420.10116859391764102No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4450.059.3198281
GTATCAA16750.053.188631
TCAACGC21800.040.5332874
ATCAACG22150.039.8928073
CAACGCA22500.039.0633625
AACGCAG23050.037.9273536
GTACATG35850.033.397691
ACCTAAG9950.033.1624641
TACATGG36350.032.713472
TATCAAC28000.032.061652
ACGCAGA27600.031.841717
ACATGGG37800.031.4585883
CGACGTA300.004163232631.3308822-23
TAAGACA12050.031.204234
CTAAGAC11650.031.0652853
CGCAGAG29300.029.5130488
CGCCGTA804.7778763E-429.37743494
CCTAAGA12400.028.8072912
GCAGAGT30750.028.4270449
TATCGAG700.008833921526.8579252
Produced by FastQC (version 0.11.2)