FastQCFastQC Report
Sun 14 Apr 2019
SRR6576909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234366
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44240.35840261316335675No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26390.21379396386485044No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21210.17182910093116627No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15370.12451736356963819No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14060.11390462796285704No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT13760.11147423049565526No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13270.10750458129922567No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12590.10199568037356829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7050.062.690411
GTATCAA25200.051.6822741
TCAACGC29900.042.125984
CAACGCA31100.040.6516655
ATCAACG31150.040.4355353
AACGCAG32200.039.4089056
TATCAAC36600.035.070662
GTCTTAG11650.033.094081
TTAGGTA9950.032.592114
TAGGTAT10000.032.4291465
ACGCAGA39100.032.213997
GTACATG48100.032.159791
TACATGG48550.031.7648642
CTTAGGT10300.031.484613
ACTACGC753.2761856E-431.332513
ACATGGG49550.030.5420823
CGCAGAG41450.030.387628
AGGTATA10800.030.0269876
TCTTAGG12950.029.4088232
GGTATAG11300.029.1142797
Produced by FastQC (version 0.11.2)