FastQCFastQC Report
Sun 14 Apr 2019
SRR6576902_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576902_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397711
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8290.20844281400313291No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6380.1604179919589853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.10661007616083035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12550.040.514821
GGGTACC2300.030.6501267
GTACTTG2500.030.1310211
GTACATG21800.030.0187231
TACATGG21450.029.8012892
ACATGGG22450.029.3076173
ATCAACG16050.029.281533
TCAACGC16300.028.8324284
ATGGGTA2950.028.676055
CAACGCA16550.028.396895
AACGCAG16550.028.396896
CATGGGT4800.028.3939344
TATCAAC17800.027.726352
GGTACCT2800.026.8553478
GGTATCA6400.025.7467251
CATGGGA13050.024.8489114
GGTATAG950.001303594124.735197
CCTATAC950.001303594124.735193
GCATATA1351.4353525E-524.4117071
ACGCAGA19350.024.287787
Produced by FastQC (version 0.11.2)