FastQCFastQC Report
Sun 14 Apr 2019
SRR6576902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397711
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6130.15413202048723823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5990.15061187646305985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA255.5557193E-575.1977844
CCGAACT602.5233021E-854.9076541
CGCTAAA352.9355974E-453.7127085
CCCGCTA352.9355974E-453.7127083
GTATCAA10900.047.4954761
ATCAACG12900.039.3476833
GGTATCA4050.038.3482061
TCAACGC13300.038.164294
CAACGCA13200.038.0973635
AACGCAG14100.035.9989436
GTACATG20900.034.9037061
TATCAAC15100.033.6614572
TACATGG21850.032.9553642
ACATGGG22150.032.2518733
ACGCAGA16000.031.1365837
CGCAGAG16150.030.8473918
GTACTTG2203.6379788E-1227.8103711
TGCCCGC856.754488E-427.684531
ATAGACG856.8093697E-427.6462448
CATGGGA11050.027.2209174
Produced by FastQC (version 0.11.2)