FastQCFastQC Report
Sun 14 Apr 2019
SRR6576897_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576897_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128260
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29330.2599578111428217No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18290.16210802474606917No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16380.14517930264300782No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16140.1430521333735132No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13680.12124864836119335No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12360.10954921737897294No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12110.10733341605658271No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11910.10556077499867052No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11570.10254728520021981No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11310.10024285182493398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.064.6438751
GTATCAA21650.058.2762031
ATCAACG28150.044.4045833
TCAACGC28500.043.6943784
CAACGCA29550.042.141795
AACGCAG30550.040.6085326
TATCAAC35900.035.080432
ACGCAGA38200.032.353187
CGCAGAG39100.031.6309038
TACATGG47000.030.1949022
GTACATG47650.030.0348221
GCAGAGT42850.028.972489
ATACGGT650.00615919728.9181946
ACATGGG49100.028.329233
GTCTTAG7200.025.500371
CATGGGG28350.025.0292844
TTATAAG950.00130046724.75021794
ACCTAAG11250.024.689591
CTTAGGT6550.024.3928283
AGAGTAC38800.024.36110110-11
Produced by FastQC (version 0.11.2)