FastQCFastQC Report
Sun 14 Apr 2019
SRR6576895_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576895_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290457
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA56860.4406190985054132No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC38190.2959416702764989No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28880.223796685980238No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18920.14661472641087614No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17890.13863305790119312No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC16430.1273192365185357No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16340.1266218091730294No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16270.12607936568208009No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT15490.12003499535435896No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15400.11933756800885267No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14740.11422310080847328No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14260.11050348829910643No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14130.10949609324448625No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13690.10608644844423332No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA13520.10476908568049924No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13250.10267680364398038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7000.060.423991
GTATCAA25550.054.6299061
TCAACGC30200.045.5870484
ATCAACG30250.045.3563653
CAACGCA31400.043.994515
AACGCAG32700.042.1017956
TATCAAC36500.037.8546642
ACGCAGA41250.033.3752447
CGCAGAG42950.032.054228
GTACATG53500.030.657491
TACATGG53450.030.4223882
AAGGGTA5300.030.1428155
ACATGGG53750.029.8970553
GCAGAGT46100.029.8639669
GGTAATC4900.029.7266758
ACCAGAT21250.029.4085494
ACCTAAG20700.028.6065271
CTAAGAC21600.028.2794113
GTAATCA6300.027.5957329
CTTAGGT11350.027.3230293
Produced by FastQC (version 0.11.2)