FastQCFastQC Report
Sun 14 Apr 2019
SRR6576891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430214
Sequences flagged as poor quality0
Sequence length100
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6670.1550391200658277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.14550897925218612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT200.002159713870.519281
GTATCAA11650.047.6181641
ATCAACG14700.036.4504663
CAACGCA15000.036.026435
TCAACGC15350.035.204984
AACGCAG15800.034.4997146
GTACATG25750.031.4027561
CGCAAAA753.2758413E-431.3273286
TACATGG25750.030.8550342
TATCAAC17850.030.2883912
GTACTTG2350.030.0082021
TCTATAC953.7458336E-529.6854233
GGGTACC3350.029.4570397
GGTATCA5000.029.1479661
ACGCAGA18850.028.9175327
ACATGGG26650.028.7478893
CATGGGT6050.028.7382934
CGCAGAG19400.028.0977068
CAAACGC1057.3971874E-526.8644852
CGTTAGC350.00883305226.85823664-65
Produced by FastQC (version 0.11.2)