FastQCFastQC Report
Sun 14 Apr 2019
SRR6576890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109933
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18040.1625323330327146No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18020.1623521419761373No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17150.15451383101502522No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15810.14244103022434687No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14250.12838612781131833No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14200.12793565016987513No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA14150.1274851725284319No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13740.12379125586859747No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC13600.12252991847255645No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13410.12081810343507221No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12110.10910568475754844No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12030.10838492053123927No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA12020.10829482500295062No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG11890.10712358313519825No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT11700.10541176809771402No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG11700.10541176809771402No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11440.10306928436220925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18600.042.9559171
TACATGG40100.034.342342
GTACATG41200.034.2224461
ACCTAAG6650.033.2172161
ACATGGG41250.032.921733
ATCAACG24550.031.7735213
CAACGCA24550.031.5806925
CCTAAGA7300.031.548582
TAGTACT5750.031.0544974
GGTATCA8850.030.801491
CATGGGG25850.030.1756254
AACGCAG27550.028.6534466
CTAGTAC6450.028.4127753
TCAACGC28300.027.7292944
ATTAGGC700.00884473826.8516333
TAGGTAT3500.026.8504225
GTACTGT7000.026.8504226
TTAGGTA3400.026.2593174
ACGCAGA29900.025.9299667
GTACTAG3650.025.7527831
Produced by FastQC (version 0.11.2)