FastQCFastQC Report
Sun 14 Apr 2019
SRR6576889_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576889_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122841
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18760.16707619333458612No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17930.15968422955698983No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16750.14917517262016616No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16480.14677055789733365No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14610.13011637444660465No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14120.12575244402368635No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14110.12566338421913698No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG14040.12503996558729152No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG13960.1243274871508967No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA13680.12183381262351482No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13180.11738082239604716No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA12800.11399654982317176No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG12670.11283877236403017No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12510.11141381549124052No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG12400.11043415764119763No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11880.10580304780463128No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11840.10544680858643386No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11670.10393279190909488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18250.049.0655671
CAACGCA22650.038.3874135
ATCAACG22900.038.173573
AACGCAG24950.035.4138036
GGTAATC1850.033.026078
TCAACGC27050.032.6644864
TACATGG39300.032.2891462
GTACATG39650.031.9738671
ACATGGG40150.031.3714473
ACGCAGA28350.030.502177
GTACTAG4100.029.8865361
CGCAGAG29050.029.7685038
TATCAAC31600.029.0023272
ACCTAAG7400.028.6593841
GTATATA2002.5465852E-1128.2772621
GTTCTAG1354.5249726E-727.9281581
GCAGAGT31400.027.3909289
AGAGTAC28150.027.38109410-11
GGTATCA7800.027.1896741
CGTAAGG700.00883718326.8563672
Produced by FastQC (version 0.11.2)