FastQCFastQC Report
Sun 14 Apr 2019
SRR6576887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382517
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA45120.3263612671670583No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29490.21330659948485264No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23050.16672489379877425No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17570.1270870448609312No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16170.11696058710308807No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15340.11095704428950966No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15170.10972740299034299No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15140.10951040746696061No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14050.10162623678406847No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13920.10068592284941161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.061.3966671
GTATCAA27350.052.7742161
TAAGTCG503.0103265E-546.9984665
ATCAACG33900.042.284753
TCAACGC34200.042.188684
CAACGCA35000.041.2243655
AACGCAG36400.039.6388136
TATCAAC42900.033.864292
GTACATG58100.031.8831211
TACATGG57200.031.727222
ACGCAGA46100.031.5022227
ACATGGG57950.031.143073
CGCAGAG47100.030.833398
GCAGAGT50650.028.8578919
GTCTTAG9500.028.2092821
CTTAGGT8050.026.8562643
ACCTAAG13800.026.573961
CATGGGG34400.026.23174
AGAGTAC45150.025.346910-11
TAGGTAT8400.025.1777465
Produced by FastQC (version 0.11.2)