FastQCFastQC Report
Sun 14 Apr 2019
SRR6576884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889666
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA50550.5681907592287443No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC32410.36429401595655No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29030.3263022302751819No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC22010.24739621386003288No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18560.208617616049169No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA13440.15106792886319134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13420.15084312539762113No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13290.14938190287141467No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA13010.14623465435343153No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT12240.13757972092897786No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT11820.13285884815200313No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT11180.12566513725375592No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10930.12285509393412809No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG10920.12274269220134298No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA10570.11880863155386405No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA10500.11802181942436824No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT10440.11734740902765758No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG10020.11262653625068283No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA9970.11206452758675726No Hit
GTATAAATTCTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAAT9230.10374679936065893No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT9170.10307238896394827No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5850.072.3204041
GTATCAA23950.054.5649151
TCAACGC29400.043.3136984
ATCAACG29650.042.948493
CAACGCA29700.042.7155725
TATCAAC30550.042.163632
AACGCAG31000.040.924276
AATAGTA5800.035.6454125
TAGTATG5600.035.240357
GTACATG47850.034.973761
ACGCAGA36500.034.7576037
TAATAGT5900.034.246784
GACGCGC550.002720330434.172468
ACGCGCA550.002721080334.1705369
TACATGG49000.033.8670432
TAGGTAT7800.033.734355
TTAGGTA8100.033.0668754
CGCAGAG38450.032.994868
ACATGGG49700.032.9022943
GTCTTAG9150.032.8800961
Produced by FastQC (version 0.11.2)