FastQCFastQC Report
Sun 14 Apr 2019
SRR6576883_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576883_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892439
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA50450.565304743517484No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC30430.3409756857331425No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26050.2918967010630419No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18770.21032249823237217No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15310.17155234139252096No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG14650.16415687794908113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12950.14510795695840276No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA12150.13614375884514235No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11940.1337906568404115No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC11890.13323039445833273No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC11450.1283000854960395No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG10680.11967204481202637No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA10130.11350915860915983No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9830.11014758431668718No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT9820.11003553184027143No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT9810.10992347936385567No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC9580.10734627240629331No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9570.10723421992987756No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9380.10510522287797823No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT9240.10353648820815764No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9060.10151954363267406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6400.070.6836241
GTATCAA24150.056.1956751
CAACGCA31600.041.0537645
TCAACGC31650.040.9912074
ATCAACG31750.040.8621033
AACGCAG32700.039.6727526
TATCAAC33500.039.1484642
ACGCAGA38300.033.743647
ACCTAAG17800.032.826851
CGCAGAG39950.032.467618
TAAGACA19450.031.9013944
TAGGTAT8300.031.7132645
TTAGGTA8650.031.5186234
CTTAGGT8650.030.9751993
CTAAGAC19100.030.5171283
AGGTATA8550.030.2362256
GCAGAGT43200.030.025029
CCTAAGA19600.029.9784562
GTACATG46300.029.9222241
GTCTTAG9550.029.6057051
Produced by FastQC (version 0.11.2)