FastQCFastQC Report
Sun 14 Apr 2019
SRR6576883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892439
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA50630.5673216880929677No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC30010.3362694817236808No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC27360.3065755754735057No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC22180.24853239269014465No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16540.1853347959916588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.1480213213452124No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA13000.14566821934048152No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA12290.13771249351496292No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC12100.13558349646306359No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT11220.12572287853847713No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG10850.12157693691109421No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT10810.12112872700543119No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10780.12079256957618391No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT10520.1178792051893743No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT10100.11317300117991258No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA9610.10768242983554058No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA9560.10712216745346181No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9340.10465701297231518No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA9110.10207980601475283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5850.062.694621
GTATCAA22500.056.8431241
ATCAACG30400.041.743323
TCAACGC30550.041.5360344
CAACGCA31050.040.8671765
AACGCAG32150.039.7612846
TATCAAC32500.039.3529322
GTACATG49550.034.637041
ACATGGG50400.033.851113
TACATGG51250.033.67162
ACGCAGA38400.033.289727
TAGGTAT8100.032.491935
CTTAGGT8050.032.1117253
CATGGGG26500.031.9226654
GTGCAAG4300.031.7118131
GTCTTAG8900.031.6995281
CGCAGAG40350.031.6809278
AAGGGTA4850.031.00855
AGGTATA8500.030.4099886
GGGTACC3250.030.3674567
Produced by FastQC (version 0.11.2)