FastQCFastQC Report
Sun 14 Apr 2019
SRR6576881_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576881_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365324
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA55800.4086942000580083No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC32850.2406022306793113No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25180.18442508884338077No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC16580.12143637700648344No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16440.12041097937192931No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15250.1116950994782191No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT14990.10979078958547568No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14470.10598216979998887No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG13850.10144112313267767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7500.066.009121
GTATCAA24200.052.9943351
ATCAACG30400.041.7426573
TCAACGC30750.041.2660264
CAACGCA32600.039.0684055
AACGCAG33200.038.362356
TATCAAC35600.035.6454122
CCCTATA1107.699418E-834.181192
CTAAGAC18000.032.638293
ACCTAAG16850.031.6194551
GTACATG56000.031.0680681
ACGCAGA41200.030.9133537
TACATGG56300.030.6370942
CGCAGAG42600.030.1180658
ACATGGG57150.030.0169473
CCTAAGA19450.029.4801792
TAGGTAT10350.029.0612075
TAAGACA22200.029.002914
CTTAGGT10550.028.5113283
GTCTTAG11750.028.4902571
Produced by FastQC (version 0.11.2)