FastQCFastQC Report
Sun 14 Apr 2019
SRR6576881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365324
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA54160.3966823991960883No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC33240.24345869551842642No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25680.1880872232525027No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17970.13161711066384243No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC16710.12238853195285515No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16150.11828694141463858No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15970.1169685730273547No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT15590.11418535087642202No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14590.10686108205817814No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14380.10532298560634691No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13850.10144112313267767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.061.551251
GTATCAA27400.056.7881471
TCAACGC32650.045.6310964
ATCAACG32750.045.4917643
CAACGCA33650.044.694055
AACGCAG35000.043.23876
TATCAAC41550.036.43052
GTCTTAG11450.035.718571
ACGCAGA42250.035.70787
TAGGTAT10800.034.8137665
CTTAGGT10700.033.8214153
CGCAGAG44800.033.7802358
TTAGGTA11300.033.273344
TAACGCT1001.4117522E-632.8990144
AGGTATA11550.031.7393076
GCAGAGT48050.031.39769
GTATAGT12650.031.2085488
GGTATAG11800.030.6685717
TATAGTA13100.030.4952689
AAGGGTA5150.030.1156025
Produced by FastQC (version 0.11.2)