FastQCFastQC Report
Sun 14 Apr 2019
SRR6576880_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576880_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461769
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7810.16913218514018916No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4710.10199905147378885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12000.042.3566061
ATCAACG15500.032.4428443
CAACGCA16050.031.6239095
TCAACGC15950.031.5275294
GTGTACG600.00413129531.3752631
CGGTCGA300.00415981131.3344936-37
AACGCAG16200.031.3310956
TACATGG22850.030.6455122
GTACATG22850.030.2767851
TATCAAC17650.029.2896942
ACATGGG23450.029.059763
TAGGCGG856.8127154E-427.6450825
ACGCAGA18800.026.9980727
CGCAGAG19000.026.9612338
TGCGTAG700.00877323626.8930841
TATAGGG909.512989E-426.1092472
CATGGGG9800.025.8961094
ATAATGC1301.08659315E-525.3058853
GGTATCA6450.024.0786911
GCAGAGT21850.023.8747259
Produced by FastQC (version 0.11.2)