FastQCFastQC Report
Sun 14 Apr 2019
SRR6576879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823609
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA70770.8592669579861317No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC44920.5454044334143993No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC44170.5362981706125115No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC33210.40322531686759133No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG22600.2744020524302187No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA19310.23445591293927096No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT19230.23348457824040292No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA18320.22243564604077906No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC17060.20713712453360758No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC16450.19973069745473884No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT16060.19499544079775719No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG15280.1855249274837939No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT14670.17811850040492516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14650.17787566673020816No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT14200.17241190904907547No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13400.16269856206039515No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA13320.16172722736152714No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG13060.15857038959020603No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT12730.1545636339573754No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA12460.1512853793486958No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT12230.1484927920894502No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12150.14752145739058217No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG11910.1446074532939781No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT11780.14302903440831755No Hit
GTATAAATTCTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAAT11760.14278620073360054No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT11630.14120778184793997No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA11560.14035786398643044No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG11390.1382937777513359No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA11340.13768669356454338No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT10920.1325871863954862No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC10280.12481650880454195No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA10210.12396659094303243No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC9850.11959558479812629No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT9610.11668158070152221No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT9570.11619591335208818No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA9300.11291765874340859No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA9260.11243199139397457No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG9150.11109640618303103No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA8900.10806098524906843No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC8730.10599689901397386No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC8710.10575406533925687No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG8700.10563264850189835No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG8580.10417564645359631No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA8490.10308289491736977No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA8380.10174730970642623No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG8250.10016889082076567No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5850.066.684071
GTATCAA27600.044.4458541
TCAACGC27700.043.08724
ATCAACG28300.042.173693
CAACGCA28350.041.9310235
AACGCAG28900.040.9704446
CGATCGA354.6440728E-640.2761162-63
TATCAAC30250.039.6200142
GTACATG42100.036.729381
GTGCAAG5550.036.4145771
ACATGGG42750.035.612563
ATGGGGG9150.035.4320265
TACATGG43950.034.86252
ACGCAGA34500.034.320177
AAGGGTA6400.033.771155
CATGGGG23100.033.766844
CGCAGAG35950.033.0666058
GCAGAGT37150.032.124989
TAGGTAT12600.031.6968675
CCTATAC902.5972655E-531.3258653
Produced by FastQC (version 0.11.2)