FastQCFastQC Report
Sun 14 Apr 2019
SRR6576873_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576873_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272937
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA52770.41455311614007606No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31690.24895183343716146No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC26220.20598034309631977No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16690.13111410855368333No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14930.11728781550068856No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14260.11202439712255988No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG14250.11194583863930421No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13990.10990331807465728No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13850.10880349930907814No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13540.10636818632815292No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT13440.10558260149559641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7150.058.590381
GTATCAA25550.053.0571561
TCAACGC30550.043.992654
ATCAACG31400.042.801763
CAACGCA32600.041.082095
AACGCAG33950.039.4484866
TATCAAC38300.035.7056162
CTTAGGT10650.035.2992633
AGGTATA10300.035.130056
TAGGTAT10400.034.792265
TTAGGTA10850.034.6485864
GTCTTAG11550.034.6401141
GTACTAG702.1661702E-433.6212841
ACGCAGA40700.032.7893037
GGTATAG11350.032.7069057
CGCAGAG42200.031.6461778
GTATAGT11650.031.4835748
TATAGTA11750.031.215639
ACCTAAG18450.030.1042631
GTACATG53200.029.9937271
Produced by FastQC (version 0.11.2)