FastQCFastQC Report
Sun 14 Apr 2019
SRR6576869_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576869_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511563
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7190.1405496488213573No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5280.10321309398842371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12900.042.7677881
ATCAACG16800.032.7272073
TCAACGC16900.032.255494
CAACGCA17450.032.046745
AACGCAG17650.031.9498566
TACATGG25300.031.9477522
GTACATG25550.031.7437461
ACCGTGC600.00416191331.328618
ACATGGG26150.030.729593
ATGGGTA2750.029.0501675
TATCAAC19350.028.4143222
ACGCAGA20900.026.9815777
CATGGGT6450.026.9571744
CTATACC1057.421275E-526.8530924
CATGGGA14950.026.0897124
CGCAGAG21550.025.9496848
ATGGGAC3850.024.4119035
CATGGGG9150.023.1112694
GGTATCA6800.022.8836691
GTACTTG3350.022.5214211
Produced by FastQC (version 0.11.2)