FastQCFastQC Report
Sun 14 Apr 2019
SRR6576869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511563
Sequences flagged as poor quality0
Sequence length100
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.14015869013200719No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5870.11474637532425136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13600.046.3079571
ATCAACG16750.037.5919463
TCAACGC17050.036.9305044
CAACGCA17550.036.1461035
AACGCAG17700.035.839786
TATCAAC18900.033.0734562
GTACGTA600.004159223831.3327451
TATGCGT600.00416320631.326626
TACATGG27450.030.3054432
GTACATG27700.030.0319291
ACATGGG27700.030.0260583
ACGCAGA21300.029.782357
CGCAGAG21350.029.7126038
GTACTTG3750.028.8261281
GTACTAA1354.6269815E-727.8513321
CATGGGG11350.026.4965274
GCAGAGT24100.026.322169
CATGGGT6250.025.5625234
GGTATCA6400.024.233921
AGAGTAC22400.022.44608510-11
Produced by FastQC (version 0.11.2)