FastQCFastQC Report
Sun 14 Apr 2019
SRR6576868_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576868_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271238
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43620.343130082643848No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC27240.2142793088312338No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20200.1589002216736756No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15110.11886051235095237No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13050.1026558362792805No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12790.10061058590130252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.052.512251
GTATCAA22250.048.8670431
TCAACGC26300.040.917864
ATCAACG27200.039.5639573
CAACGCA27400.039.4466745
AACGCAG29100.037.142236
GGTACCT5950.032.4033978
TATCAAC33750.032.309692
GTACATG48150.032.0635531
ACCTAAG16450.032.0469131
TACATGG47850.031.7277412
CTAAGAC17850.031.5919323
TAAGACA20200.030.9409374
ACGCAGA36250.029.8162487
ACATGGG50300.029.8027173
CCTAAGA18700.029.407822
CGCAGAG37800.028.7371588
TGGGTAC7300.027.6808096
TATACCG1606.927257E-826.4335614
GGGTACC7300.026.393337
Produced by FastQC (version 0.11.2)