FastQCFastQC Report
Sun 14 Apr 2019
SRR6576867_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576867_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320847
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA42450.3213846872499237No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26220.19850898703634864No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20490.15512773243229536No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15130.11454771067352994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7300.054.8907051
GTATCAA24350.050.5294151
TCAACGC28800.041.2885784
ATCAACG29650.040.2634433
CAACGCA29750.039.8121345
AACGCAG31400.038.019466
TATCAAC35350.034.1700482
GGTCGAG753.2770802E-431.3312518
ACGCAGA38650.031.0069967
GTACATG53450.030.6926421
TACATGG54050.030.0002772
CGCAGAG40250.029.774428
ACATGGG55650.029.2221933
TTAGGTA8150.028.2579294
GTCTTAG9200.027.6699681
TAGGTAT8000.027.6127555
TATACGA700.00883609126.8573912
CTTAGGT8400.026.8573913
GCAGAGT45400.026.3969259
AGGTATA8550.026.386216
Produced by FastQC (version 0.11.2)